Katalog der Deutschen Nationalbibliothek

Skin Tissue Engineering : Methods and Protocols / edited by Thomas Biedermann, Sophie Böttcher-Haberzeth

Thomas Biedermann (Herausgeber)
Sophie Böttcher-Haberzeth (Herausgeber)
SpringerLink (Online service) (Sonstige)

New York, NY: Springer US, Imprint: Humana
2025

2nd ed. 2025

Online-Ressource, XVI, 358 p. 106 illus., 96 illus. in color. : online resource.

Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture -- Isolation and Culture of Epidermal Melanocytes -- Initiating Separate Cultures of Keratinocytes and Melanocytes from a Single Biopsy -- Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor -- Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells -- Isolation and Culture of Human Dermal Fibroblasts -- Isolation and Culture of Human Dermal Microvascular Endothelial Cells Jennifer Bourland -- Isolation of stromal vascular fraction by fractionation of adipose tissue -- Engineering a multilayered skin equivalent: the importance of endogenous extracellular matrix and model flexibility for nuanced and accurate skin studies -- Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes -- Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models -- Engineering a pigmented skin equivalent that is responsive to external stimuli -- Bioprinting a tri-layered skin analogue -- Transdifferentiation of human dermis derived fibroblasts into iNeurons and cultivation in a collagen type 1 hydroge -- Deep proteome profiling of primary skin fibroblasts -- Characterization of human skin derived cells by Raman micro-spectroscopy -- Standard Preparation Protocol of Human Skin and Skin Substitute Samples for Transmission Electron Microscopy -- Methods for assessing scaffold vascularization with human endothelial cells -- Histological and histochemical methods for the evaluation of tissue engineered skin substitutes -- Pig Model to Test Tissue engineered Skin -- Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model -- Ex vivo human skin model to study immediate and early local responses to external stimuli -- A Method to Evaluate Skin and Skin Substitute Mechanical Properties Using a Suction Device -- GMP compliant production of therapeutic components of autologous adipose tissue -- Quality controls of autologous skin products according to European GMP guidelines -- Standardized outcome measures for the clinical application of tissue engineered products

978-1-0716-4510-9

Englisch

Deutschland

Medizin, Gesundheit
Biowissenschaften, Biologie

Hautzelle, Tissue Engineering, Hauttransplantation

617.4770592

Methods in Molecular Biology ; 2922

urn:nbn:de:101:1-2504120436551.951998304336 (URN)
10.1007/978-1-0716-4510-9 (DOI)

1362907375